Thermo Fisher LTQ-Orbitrap Elite
The Thermo Fisher LTQ-Orbitrap Elite combines the newest high speed dual linear ion trap and S-lens technology in the LTQ Velos, with the Orbitrap mass analyzer, providing high mass accuracy and resolution for both MS and MSn spectra. This instrument is typically run with the Orbitrap acquiring full MS data at low ppm mass accuracy and resolution up to 120,000 HWFM, while the LTQ acquires collisionally induced dissociation (CID) MSMS scans of peptides for identification and modification site determination. Other operational configurations include SRM/MRM experiments, and high energy collisions (HCD) with high accuracy MSMS spectra in the Orbitrap. Two Elite instruments are currently running in our facility at this time, both configured with capillary chromatographic and nanospray ESI interfaces.
Waters nanoACQUITY UPLC
Driving the chromatography on both of the Orbitraps is the Waters nanoACQUITY UPLC system. This system maintains accurate gradient production in a splitless configuration in the sub-μl range at pressures up to 10,000psi. The autosampler allows automated trapping for sample concentration and desalting and provides excellent reproducibility and linearity across the injection volume range of 0.1 to 20μl. Custom parts acquired from ESI Source Solutions http://www.esisourcesolutions.com.
Applied Biosystems Procise 494-HT
Edman sequencing is a classic protein characterization technique that has been a mainstay in this laboratory for over 20 years. This process requires >1pmol of protein, either in solution of on PVDF blot, and can quantitatively determine the sequence of one or several protein or peptides with a cycle time (one amino acid per cycle) of about 45 minutes. This is not a database dependent technique, so it can be considered the original form of denovo protein sequencing. Typical sequences are 10 amino acids, but extended sequence runs can be performed for novel proteins or peptides.
Thermo Fisher Q Exactive™ HF Hybrid Quadrupole-Orbitrap Mass Spectrometer
Identify and quantify more proteins, peptides, lipids, glycans and small molecules accurately and in less time with the Thermo Scientific™ Q Exactive™ HF hybrid quadrupole-Orbitrap mass spectrometer. The Q Exactive HF system combines a state-of-the-art segmented quadrupole for high-performance precursor ion selection with a high-resolution, accurate-mass (HR/AM) ultra-high-field Orbitrap mass analyzer to deliver a superior combination of scan speed, resolving power, mass accuracy, spectral quality and sensitivity. Identify, quantify and confirm in a single analysis with a single instrument with the Q Exactive HF mass spectrometer.