#  Protocols 

 



**Gel Quantity Estimation**

A reasonable estimation of protein quantity is essential to the success of a project. We have found one of the best ways to do this is by gel band size and intensity, using this **Quantity Estimation Protocol.**

**In-Gel Sample Preparation**

For preparing in-gel samples for digestion and identification by mass spectrometry, we have a detailed protocol which covers all the bases for basic protein ID work, follow the protocol for[ ](/files/prep_for_in_gel_digestion_120403.pdf)**In-gel sample preparation protocol**.

 **Chloroform/Methanol protein precipitation**

For isolating proteins from Lysed cells either thru our Covaris Lysis instrument or Lysis buffers. Follow the **Protocol for Chloroform/MeOH Precipitation**



 

 Harvard Center for Mass Spectrometry Proteomics Portal



 

 

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 Attachments- [  picture\_as\_pdf  quantity\_estimation\_protocol\_120403.pdf ](/sites/g/files/omnuum7266/files/msprl/files/quantity_estimation_protocol_120403.pdf)
- [  picture\_as\_pdf  In-gel\_preparation\_protocol.pdf ](/sites/g/files/omnuum7266/files/msprl/files/gel_preparation_protocol_01.pdf)
- [  description  cholorform\_meoh\_precipitation.docx ](/sites/g/files/omnuum7266/files/msprl/files/cholorform_meoh_precipitation_140930_01.docx)
 
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