Protocols

At the heart of every project is a detailed sample preparation discussion with one of the staff scientists. Once the project has been approved, samples may be prepared following these protocols.

Firstly, a reasonable estimation of protein quantity is essential to the success of a project. We have found one of the best ways to do this is by gel band size and intensity, using this Quantity Estimation Protocol.

 

For preparing samples for Edman sequencing (aka N-terminal or chemical amino acid sequencing) please follow this protocol to maximize your chance of the highest quality results, use this Edman sample prep protocol.

 

For preparing in-gel samples for digestion and identification by mass spectrometry, we have a detailed protocol which covers all the bases for basic protein ID work, follow the protocol for In-gel sample prep.

 

More detailed projects, such as modification site determination, mapping, GeLC and quantitative analysis demands an in-depth discussion well in advance of the actual sample prep. 

quantity_estimation_protocol_120403.pdf126.57 KB
edman_chemical_sequencing_sample_prep_120403.pdf119.38 KB
prep_for_in_gel_digestion_120403.pdf174.27 KB