Protocols

Gel Quantity Estimation

A reasonable estimation of protein quantity is essential to the success of a project. We have found one of the best ways to do this is by gel band size and intensity, using this Quantity Estimation Protocol.

Edman Sequencing Protocol

For preparing samples for Edman sequencing (aka N-terminal or chemical amino acid sequencing) please follow this protocol to maximize your chance of the highest quality results, use this Edman sample prep protocol.

In-Gel Sample Preparation

For preparing in-gel samples for digestion and identification by mass spectrometry, we have a detailed protocol which covers all the bases for basic protein ID work, follow the protocol for In-gel sample preparation protocol.

 

 

quantity_estimation_protocol_120403.pdf126 KB
edman_chemical_sequencing_sample_prep_120403.pdf119 KB
prep_for_in_gel_digestion_120403.pdf174 KB