Gel Quantity Estimation
A reasonable estimation of protein quantity is essential to the success of a project. We have found one of the best ways to do this is by gel band size and intensity, using this Quantity Estimation Protocol.
In-Gel Sample Preparation
For preparing in-gel samples for digestion and identification by mass spectrometry, we have a detailed protocol which covers all the bases for basic protein ID work, follow the protocol for In-gel sample preparation protocol.
Chloroform/Methanol protein precipitation
For isolating proteins from Lysed cells either thru our Covaris Lysis instrument or Lysis buffers. Follow the Protocol for Chloroform/MeOH Precipitation