Gel Quantity Estimation

A reasonable estimation of protein quantity is essential to the success of a project. We have found one of the best ways to do this is by gel band size and intensity, using this Quantity Estimation Protocol.

In-Gel Sample Preparation

For preparing in-gel samples for digestion and identification by mass spectrometry, we have a detailed protocol which covers all the bases for basic protein ID work, follow the protocol for In-gel sample preparation protocol.

 Chloroform/Methanol protein precipitation

For isolating proteins from Lysed cells either thru our Covaris Lysis instrument or Lysis buffers.  Follow the Protocol for Chloroform/MeOH Precipitation


quantity_estimation_protocol_120403.pdf126 KB
cholorform_meoh_precipitation_140930.docx25 KB
prep_for_in_gel_digestion_120403.pdf174 KB